Preparation of Boer Goat Semen Diluent and Glass Bottle Packaging Technology Jiang Hua, Jiang Feng 2, Chen Yakang 2, Ni Hongxiang 2 (1. Haimen City Animal Husbandry and Veterinary Station, 226100; Jiangsu Haimen Livestock Improvement Station) in the introduction of Boer In the process of accelerating the industrialization of goats, the meat goats encountered the problem that the number of rams in the Boer goats could not meet the production needs. In response to the above situation, after a period of trials, we have developed a dilution formulation suitable for the preservation of Boer goat semen and a small glass bottle packaging technology. After use, the semen preservation time is prolonged, the semen utilization rate is improved, and the contradiction between semen supply and demand is alleviated. The technique is now briefly described below.

1 Diluent Preparation 1 The required reagents are of analytical grade and the water is double distilled water.

1.2 Formulation ratio Take 100ml dilution solution as an example: glucose 5.20g lactose 2 00g trisodium citrate 0. 35gEDTA (ethylenediaminetetraacetic acid) 0 05g add water to 100ml dilution pH 7. (0 can be added in winter Appropriate egg yolk.

1.3 Sterilization and disinfection are firstly filtered by conventional methods, then filled with salt water and tiled, and placed in a pressure cooker for 10 lbs for 10 minutes for disinfection.

2 glass bottle small packaging operation method 1 material preparation 6cm medical glass vial, the plug is a medical non-toxic rubber stopper, as a semen dispensing container.

~5cm flat, smooth fir, surrounded by a margin of about 2cm, and then put a small round hole with a smooth edge on the fir block, the number is 5 rows, 20 columns and 100 holes, the hole diameter is 0. The distance from the center of the hole is 1.5cmX1.8cm Can be used as a glass bottle fixing and dose scale device. The size and number of holes of the fir block can also be adjusted according to needs, and should be convenient for operation and disinfection.

One ordinary straight dropper with a rubber head is used for drip semen; one 100ml beaker is used for semen dilution.

One cotton bag for heat preservation is used, and the size depends on the number of bottles.

The above equipment is cleaned, disinfected and dried according to the conventional method. 2.2 Operation requirements and steps From the beginning of the operation to the end of the dispensing, it must be a sterile operation.

Insert the vial into the hole of the wooden block, leaving no gap at the bottom, and then put it in a 40*C incubator along with other required equipment for use.

In a 100ml beaker, according to the semen density and sperm motility, it is diluted step by step with the isothermal dilution of semen 2~3 times, and the final dilution factor can be increased to 20~40 times (the sperm concentration after dilution is not less than 50 million). /ml) Drip the diluted essence into a vial with a straight dropper, so that the liquid surface just exceeds the plane of the wooden block (about 05ml) - the dropper can be sucked once to make 2 bottles.

The stopper is rotated into the bottle in turn, so as not to fall out. Put into a cotton bag, store it in the refrigerator 5*C freezer or directly load it into the insulated barrel with cold source and send it to each fine unit.

3 Survival time and effect 1 5C refrigerator storage September 5, September to September 24, 1998, 4 batches of semen (40-fold dilution) sperm viability test results show that after 5 days of storage, the vigor is still above 0.4 , basically died on the 8th to 9th day.

3.2 Indoor normal temperature preservation In October 1998, 13æ›°~æ›°, the 41st and 361nd two Boer goat semen were diluted 40 times and packed, and 30 bottles of semen were placed in the laboratory drawer to observe the sperm vitality and preservation. In 3 days, the vitality still reached 0, 6 and basically died on the 8th day.

3.3 After use, the technicians of township and town animal husbandry and veterinary stations generally believe that it is convenient to use one vial for one serving and one bottle for the first ten, and that several technicians can go to the sheep farm to insemination at the same time.

At the same time, the phenomenon that the semen is used up after the start of the bottle is not wasted, and the semen utilization rate and work efficiency are improved.

4 Summary and discussion of the water required for this dilution, higher than previously required. After the preparation of the dilution, the pH should be adjusted according to the water quality of each place.

In small packages, the dispensing time is extended and the operator needs to be added. At the same time, the indoor environmental temperature requirement is above 20C. Otherwise, the low temperature season may cause the difference between the temperature of the equipment and the temperature of the semen to be too large, affecting the vitality of the sperm.

After the sub-package dose is changed to a small extent, it is easily affected by changes in the ambient temperature. Therefore, the requirements for the thermal insulation conditions of the intermediate links such as transportation and carrying before insemination are also increased accordingly, and it is necessary to ensure that the semen temperature is relatively constant.

The township and town animal husbandry and veterinary stations have relatively poor storage conditions for a variety of reasons. If you want to use the semen from the previous day, you must first check the sperm vitality before the delivery. Only qualified ones can be used to ensure the fetus in one episode.

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